Forensic Deoxyribonucleic Acid: History and Applications

Forensic Deoxyribonucleic Acid: History and Applications Paulean Gonzalez Deoxyribonucleic acid (DNA) is located in the nucleus of every cell and is the genetic material that makes up cellular organism and viruses. However, DNA can be used in multiple ways when it comes to forensics. It assists in linking an individual to a criminal act, to exonerate victims or even to identify victims in mass disasters. DNA is what accounts for genetic information and DNA also codes for the proteins that are necessary for our body to survive. The history of DNA dates back to Gregor Mendel as well as Francis Crick and James Watson. DNA can be found in many different parts of the body but none of that would really matter if forensic units had no way of determining and linking the DNA to certain individuals which is why the multiple ways of DNA testing are so important. The inheritance of characteristics as well as the functions of DNA also plays an important role in forensic DNA. While what could be considered the most important part to how forensic DNA is used is the outcomes and the ways it was used in courts. History Gregor Mendel It was in 1865 that Gregor Mendel, who was an Austrian monk, went before the Brno Natural Science Society and presented two lectures that summarized his experiment results on heredity in the garden pea. Mendel’s experiment was based off the cross pollination of a wrinkly green pea plant with a smooth yellow pea plant. In this experiment Mendel was able to discover that there was both a dominant and a recessive trait. His experiment allowed him to come up with three different conclusions. His three conclusions were that the inheritance of each trait was determined by what is known as a gene, that an individual receives one set of genes from each parent, and that even though a trait does not show up in an individual it is possible to still be carried on to the next generations. In Mendel’s experiment he discovered what is known as the Principle/Law of Segregation or otherwise known as his First Law. In this law there was four different parts that were included. The first part was that there were other forms of the genes that were inheritable known as alleles. The second part was that each offspring receives one allele from each parent. The third part both the sperm and the egg hold one allele for each trait and during fertilization they pair. Lastly, if the alleles are different only one appears while the other is not. The one that is shown is the dominant trait while the one that is not shown is the recessive trait. Mendel also came up with what is known as the Principle/Law of Independent Assortment which is also known as Mendel’s Second Law. In this principle, Mendel discovered that the different alleles were passed on individually and not based on one other. Mendel saw many different combinations which meant that there was separation from one another. In the early 1900’s it was believed that inheritance was fluid in nature, which was termed â€Å"half-blood† and â€Å"true-blood.† However due to Mendel’s experi ment and his repeat experiments, it was discovered that the genetic information was a particulate, which is the â€Å"unchanging nature of the DNA molecule that allows DNA fingerprinting† (Herrero, 2009, p. ). Crick and Watson Roughly around 50 years ago Francis Crick, an English graduate student, and James Watson, an American post doctorate researcher, first proved that the structure of DNA was a double helix. For their efforts Crick, Watson, and Maurice Wilkins were awarded the 1962 Nobel Prize in Medicine for their â€Å"discoveries concerning the molecular structure of nucleic acid and its significance for information transfer in biological material† (Herrero, 2009, p. ). Both Watson and Crick have helped in the basic understanding of manipulating DNA as well as the understanding of DNA replication, transcription, and translation. They believed that DNA was shaped like twisted ladder. The sugar phosphate backbone made up the sides of the ladders while the nitrogenous bases made up the rungs of the ladder. The ladder of DNA is made up of building blocks that are known as nucleotides. DNA makes up every chromosome and gene and is a polymer of repeating units that are known as nucleotides. Each of the nucleotides contains three specific parts; the phosphate group, sugar, and the nitrogenous base. The nitrogenous base consists of one of the four molecules including adenine, guanine, thymine, and cytosine (Herrero, 2009). Types of DNA There are also two types of DNA’s. The two types of DNA’s are the nuclear or chromosomal DNA which is inherited from mother and father and the mitochondrial DNA which is inherited from only the mother. DNA can be located in the cells of our body. Nuclear DNA can be present in a cell’s nucleus and is a combination of information that is inherited from both parents. It helps in the makeup of an individual’s genetic material. Nuclear DNA is best known as the â€Å"molecule of life and contains the genetic instructions for the development of all living organisms† (Nuclear DNA, n.d). Nuclear DNA can be found in almost every single cell except for red blood cells. Nuclear DNA was clearly expressed in Gregor Mendel’s Pea Experiment. His experiment was able to present information to back up the statement that half the information that was received was from the mother while the other half of information was received from the father. Mitochondrial DNA does not come from cell nucleus; it instead is located in the mitochondria of the cell. The mitochondrion is a â€Å"specialized subunit within a cell that functions as the powerhouse of the cell† (Herrero, 2009, p. ). It is more likely that a small sample of mitochondrial DNA could be discovered compared to nuclear DNA. This is because mitochondrial DNA is present in hundreds to thousands of copies in each cell compared to the only two nuclear DNA copies that are present in a cell. This means that all muscle, bone, hair, skin and many other body fluids are capable of finding mitochondrial DNA. The advantages to using mitochondrial DNA are that they are more sensitive which means less DNA is needed, degrades slower than nuclear DNA, and it can be used in cases where nuclear DNA cannot. The disadvantages to mitochondrial DNA are that all people of same maternal line will be indistinguishable and there is more work to be done, it is more time consuming, and it is more costly (Herrero, 2009). DNA Tests The two previous methods that were used were the Restriction Fragment Length Polymorphism (RFLP) Analysis and the Polymerase Chain Reaction (PCR). While the new method that is currently being used is the Short Tandem Repeat (STR). Although all tests may be different, they all require that an extraction of DNA from the sample. All three tests have had their fair share of helping the forensic units as well as being the go-to test that was responsible for the prosecution of many felons as well as the exoneration of many former criminals. Restriction Fragment Length Polymorphism RFLP was the first DNA profiling technique that was used and was seen in a widespread of areas. It was developed by Sir Alec Jeffreys in 1984. This test was capable of allowing the fragments of DNA to be measured. However as it became difficult and expensive as well as taking weeks to perform and being very demanding it became outdated. For this test analysts linked the size of fragments from a reference that was known to a crime scene sample that would allow them to match two DNA profiles. One thing that caused problems for this type of testing was that many samples from the crime scenes appeared to be too small to be tested. For example a speck of blood would be of no use with the RFLP testing. Due to biotechnological advances there would soon be more sensitive tests that would turn into what is today’s DNA profiling (Herrero, 2009). Polymerase Chain Reaction The PCR test was invented so that multiple copies of a small DNA could be replicated. This test was created in 1987 by Kary Mullis, a biochemist, and Henry Elrich, a nuclear biologist. The way that this test works is that an enzyme was found that could withstand the het needed to â€Å"unzip the two strands of the DNA double helix for replication without breaking apart the enzymes itself† (Herrero, 2009, p. ). A problem that regularly occurs with the PCR test can be contamination. When the samples are being amplified it is crucial to be very careful and avoid adding extra cells onto the sample before it is amplified. A simple sneeze to a laugh could ruin the evidence (Herrero, 2009) Short Tandem Repeat By 1998, the FBI created a more multiplex version of the PCR test known as the Short Tandem Repeat (STR). This test is still being used today. Similar to the PCR test, the STR test is capable of working with small samples. There are three different color dyes that are used to â€Å"distinguish STR alleles with overlapping size ranges† (Herrero, 2009). The STR test evaluates specific regions within nuclear DNA. It is color coded as well as automated and computerized which makes it so easy to navigate (Herrero, 2009). Combined DNA Index System In 1998 the Federal Bureau of Investigation (FBI) launched the Combined DNA Index System (CODIS). Today, 44 of the 50 states are allowed to collect DNA from all felons, 28 of the 50 states are allowed to collect DNA from juvenile offenders and 39 of the 50 states can collect DNA from those who commit certain misdemeanors. In 1994, Congress came up with the DNA Identification Act of 1994 which authorized the FBI to maintain a national database that allowed the sharing of DNA information between states. There are three tiers to CODIS, which are the local (LDIS), state (SDIS), and national (NDIS). CODIS uses 13 different DNA regions that can vary from person to person and matches are searched for at more than one location on a genome for more accurate results. By 2004, all 50 states were connected with the limited profiles of those who had been convicted of serious, violent crimes. On October 30, 2004, President George W. Bush signed the Justice For All Act that expanded the CODIS system and allowed the collection of DNA from all federal felons and allowed the states to upload the profiles of anyone who had been convicted of a crime (Herrero, 2009) Inheritance of Characteristics The many different characteristics that we inherit are in the form of DNA. However, we do not inherit the characteristic; we simply inherit the information that produces our characteristics. Half of the genetic material that we inherit is received by each parent. The sperm and egg are produced in the gonads of the parents and results in the â€Å"production of gametes that carry only half of the DNA that made the parents unique† (Herrero, 2009, p. ). A new individual is created when the sperm and egg are bonded. Much like the parents this new individual has two copies of all the genetic information and can produce eggs that will only have one copy of each gene if a female; however if it is a male it also has two copies of all the genetic information but can only pass one of the two to each of his offspring. The information that we receive is in pairs. The reason for this as stated before is because half the information is received from the mother while the other half of inform ation is received from the father (Herrero, 2009). DNA in Courts Due to fact that DNA can be found in blood, semen, saliva, urine, hair, teeth, bone and tissues, it plays an everyday role in courts. Kirk Bloodworth On March of 1985, Kirk Bloodworth had been convicted of the killing as well as sexual assault of a little nine year old. The little girl’s body was found dead in July of 1984. It had been discovered that she had been beaten with a rock, strangled and raped. Bloodworth was arrested based off an eye witness stating that they had seen him with the little girl earlier on the day that she went missing. Five eyewitnesses were even able to identify Kirk Bloodworth based off sketches. Other evidence that was presented against Bloodworth was that on the day of the incident he had told his wife that he had done something that would change their lives forever as well as him mentioning something about a bloody rock (Know the Cases-Kirk Bloodworth, n.d.). Bloodworth appealed the decision. His reason for appealing was that the bloody rock had only been mentioned because during interrogation the police had shown him a bloody rock. The incident in which he told his wife their lives would change forever had been dealing with him forgetting to buy the food she had requested. The police also did not inform the defense that there was a possibility that there was another suspect. The appellate court decision led to Bloodworth being convicted and also sentenced to two life terms that would run consecutively (Know the Cases-Kirk Bloodworth, n.d.) â€Å"In 1992, the prosecution agreed to DNA testing to be performed by the Forensic Science Associates† (Know the Cases-Kirk Bloodworth, n.d., p. 1). The victim’s shorts and underwear, a stick that been discovered at the scene, as well as an autopsy slide had been compared to Bloodworth. The PCR testing that was used was able to determine that the evidence left on the underwear did not match with Bloodworth. The same tests were then performed again by the Federal Bureau of Investigation and the same results were found (Know the Cases-Kirk Bloodworth, n.d.). In June of 1993, Bloodworth was released from prison and in December of 1992 he was pardoned. Over eight years of his life were lived in prison with two of those years him facing execution. Bloodworth became the first person to be â€Å"exonerated from death row through post-conviction DNA testing† (Know the Cases-Kirk Bloodworth, n.d., p. 1). Kirk Bloodworth’s case set the stage for many cases that are soon to come (Know the Cases-Kirk Bloodworth, n.d.) Freddie Peacock After serving five years in a prison in New York and nearly three decades after being released on parole, Freddie Peacock was able to clear his name thanks to DNA testing. Peacock was the 250th person cleared through DNA testing after being convicted for crime in which he did not commit (Know the Cases-Freddie Peacock, n.d.). The crime in which he had been serving had occurred on a night in July of 1976. A New York woman was returning to her apartment from work and was attacked. When the woman was unlocking her apartment door, the man approached her from behind, took her keys and threw her to the ground. Where she was thrown to the grown she struck her head. The woman was told that if she screams she would be killed. The woman was pulled by her perpetrator to the side of a house that was nearby and was raped. Once the perpetrator was done he returned the woman her keys and left. The woman went back to her apartment building where she told the building superintendent who then proceeded to call the police. She later testified that she was only able to see her perpetrators face when she was in a dark are. She was able to describe that her perpetrator was an African-American man who weighed about 150 pounds and had been wearing a white, flower shirt (Know the Cases-Freddie Peacock, n.d.). The woman had originally struggled to remember the details of the crime but later admitted that she believed that her neighbor was the perpetrator. The superintendent responded by asking if it was Freddie and she had said yes. Peacock’s photo was included in a 10-photo collection where she identified him again. Two hours after the attack, Peacock was arrested. The woman once again identified him through a window in one-person show up procedure. After being interrogated for two and a half hours, he had initially denied being the perpetrator but the police claimed he confessed. Peacock confessed to having several severe mental illnesses and was hospitalized for it multiple times. Peacock was unable to tell authorities how, when, and where was raped. Peacock never signed the alleged confession that the officer wrote with all the details to the crime (Know the Cases-Freddie Peacock, n.d.). At the trial, the victim testified saying that she knew he was a perpetrator because of his beard and eyes and claimed that deep down she knew it was him. She claimed that Peacock and she had only spoken twice and he had entered her apartment once uninvited. A doctor even testified that the bodily samples that were collected from the victim at the hospital, but other evidence was not presented. Peacock was eventually convicted and sentenced to up to 20 years in prison (Know the Cases-Freddie Peacock, n.d.). Peacock had been released on parole after five years in the New York prison. Thanks to his families and church support he was able to receive treatment for his mental illness. After contacting The Innocence Project in 2002 to help him clear his name. DNA evidence was obtained and able to rule him out. This evidence was taken before a judge. A state judge tossed out the conviction. This meant his name had been cleared after 34 years of wrongful convictions (Know the Cases-Freddie Peacock, n.d.). Conclusion All types of cells in the body contain the copy of the same DNA, for example DNA can be found in the blood cells, saliva cells, tissues cells and even the semen cells. DNA Functions DNA has two primary functions. These two functions include transmitting information from one generation to the next as well as providing the blueprint for making proteins the same way every time. References Herrero, S. (2009). Forensic DNA: Technology, Application, and the Law. In S. H. James J. J. Nordby (Eds.), Forensic Science: An Introduction to Scientific and Investigative Techniques (pp. 303-325). FL: CRC Press. Know the Cases-Freddie Peacock (n.d.). The Innocence Project. Retrieved from http://www.innocenceproject.org/Content/Freddie_Peacock.php Know the Cases-Kirk Bloodworth (n.d.). The Innocence Project. Retrieved from http://www.innocenceproject.org/Content/Kirk_Bloodsworth.php Nuclear DNA (n.d.). Retrieved from http://en.wikipedia.org/wiki/Nuclear_DNA

Food Fight Essay -- Health Culture Nutrition Essays

Food Fight In America, one would be hard pressed to find a town which did not support at least one McDonald’s, Burger King or Wendy’s. Pizza parlors are a necessity in college towns. Ice cream shops are abuzz with customers of all ages after dark. And hey, who ever heard of a movie without popcorn? The increasing visibility and importance of food in our culture has been a phenomenon. Food began as a necessity of life. It was the source of energy, which allowed the body to grow and prosper, and for hunters and gatheerrs to survive. In modern times, the role of food in everyday life has taken on a life of its own, from the blue-ribbon palate pleasers tickling taste buds to political statements drawn in chocolate to social drinking. The resulting uses of food are as diverse as the different foods it encompasses and as inseparable from a person’s daily life as breathing. How can something so simple as energy-intake convey have become such a large and varied part of everyday life? Food as a family affair is largely determined by the family’s lifestyle; conversely, knowing a family’s eating patterns says a lot about their lifestyle. Sitcoms portray meals as a leisurely occasion for the family to sit down together to enjoy savory foods fresh from the oven, which the loving wife has been tending for the past two hours. Then reality sinks in. The increasing popularity of TV dinners, microwave gourmet, and prepackaged snacks caters to a family without the time for such luxury. Why cook when you can heat up a frozen entrà ©e of teriyaki beef or homestyle apple pie in just four minutes? As the tendency for both spouses to be employed full-time has increased from 33% of families in 1972 to 67% in 1998, according to the National Data... ...heir lives before they can begin to conquer the problems which have arisen from it. As food has become increasingly available, society has found new ways of playing with it, spinning out brand loyalty, hobbies, programming and cults dedicated to food. Before people embrace these new food novelties, however, they must first as a culture establish the terms of their new relationships with food. Only then can we both enjoy the delights of the kitchens without destroying our health and self-image in the process. Sources Cited foodies.com forfoodies.com Eric Schossler's Fast Food Nation (2001) â€Å"Body Image Statistics† womensissues.about.com/cs/bodyimage/a/bodyimagestats.htm â€Å"Overweight and Obesity Fact Sheet† www.surgeongeneral.gov/topics/obesity/calltoaction/fact_adolescents.htm â€Å"The Emerging 21st entury† cloud9.norc.uchicago.edu/dlib/sc-42.htm.

Pioneers, oh pioneers

How does the setting underline the main conflict and the meaning for characterization? Table of Contents 1. Introduction 2. Setting 2 2. 1 Dominica 3 2. 2 Dr Cox's house 4 2. 3 Ramage's house 5 2. 4 Comparison Imperial Road and Market Street 7 3. Conclusion 8 4. Bibliography 10 5. Honesty Statement 11 This seminar paper analyses the different functions of the setting in Jean Rhys's short story â€Å"Pioneers, Oh, Pioneers. † The author Jean Rhys was born in 1890 and brought up in Roseau, Dominica (Rhys 1981: 10).Her father was Welsh and her mother Creole (Rhys 1981 :6), so she grew up etween two worlds. Savory underlines that this is manifested in her divided attitude towards cultural identities (1998: 35). Rhys was an author of novels, short stories and an autobiographical fragment which is called â€Å"Smile Please†. But not only this book is autobiographical. Jean Rhys had almost always some autobiographical aspects in her stories. To her friend David Plante, who lat er became the ghostwriter of her autobiography, she said: â€Å"l can't make things up, I can't invent.I have no imagination. I can't invent character. I don't think I know what character is. I Just write about what happened† (1983: 52). In â€Å"Pioneers, Oh Pioneers†, which was originally published under the name â€Å"Dear Darling Mr Ramage† in The Times and later became a part of the story collection â€Å"Sleep It Off Lady' (Rhys 1976), the reader will also find some autobiographical aspects. The female child protagonist Rosalie is â€Å"aged 9† (Rhys 1970: 12)1 and â€Å"her father is the local doctor† (Hooper 2005:122).In 1899 Rhys was the same age and her father also had â€Å"decided to become a doctor† (Rhys 1981 : 68). Malcolm and Malcolm point out that Ramage â€Å"is neither white nor black† Just like Jean Rhys was. In her family she was the one with the palest skin (Rhys 1981). Do you consider yourself a West Indian? â €  She shrugged. â€Å"It was such a long time ago when I left. † â€Å"So you don't think of yourself as a West Indian writer? † Again, she shrugged, but said nothing. â€Å"What about English? Do you consider yourself an English writer? † â€Å"No! I'm not! I'm not!I'm not even English (Plante 1983:44). Jean Rhys seemed nowhere to be fit. To come to terms with this experiences, Rhys uses the character Mr Ramage in â€Å"Pioneer, Oh, Pioneers†. She adopts his eccentricity, but apart from that, she â€Å"gives im a rather different story' (Hooper 2005: 122). All references from the short story are from: Rhys, Jean. â€Å"Pioneers, Oh, Pioneers. † Sleep it Off Lady. London: Penguin Books, 1979, 11-21. The following analysis will start with a short definition of the term setting and a closer investigation of the general setting in the short story.Furthermore, this seminar paper is aimed to show the link between nature and the emotional state of the main character Mr Ramage. Besides, the main issue of the story, namely the cultural clash between the black and the white insulars, will be analyzed. To get a general nderstanding of the main topic of this seminar paper, namely the setting of the short story â€Å"Pioneers, Oh, Pioneers†, the definition of Michael J. Toolan is to be considered. He points out that â€Å"We like, in our reading of narratives, to know where we are, and look for clear spatiotemporal indications of Just where and when a thing happened. (1988: 102). In answer to the question of the point in time during which the action of the plot takes place, the reader finds out that â€Å"It was still the nineteenth century, November 1899†³ (11). According to Malcolm and Malcolm this date indicates he inability of Ramage to fit in the community of the island population. It symbolizes the situation of Ramage being caught between two cultures (1996: 85). He is neither Creole, nor a proper English man, b ecause he is trying more and more to disassociate himself from England, for example by marrying Isla. Her given name, Isla, the Spanish for Island, may suggest that Ramage has embraced the island itself† (Hooper 2005: 124). The date 1899 also implies that the action occurs at the time of colonialism. To have an overview of the history of the society living there at that time, the reader may onsider that the West Indies were discovered as a place where tobacco and sugar grew very well and one might earn money by planting there and shipping the goods apart. For this reason thousands of people from Africa were departed to the West Indies to work there as slaves.Although in 1899 slavery was already abolished, racial segregation was still present. â€Å"[T]he majority of Dominica's population is of African descent, mainly speaking French Creole and of the Catholic faith. † (Savory 2009: 2) but the minority of white people, mostly from Europe, who believed in the Anglican Chu rch, was still the dominating class. They urged the black people to adapt to their culture and rules (Smith 1974: 5-6). Besides telling the reader that the story is set in the time of colonialism, the date fulfils another function.November 1899 is the turning point between two seasons, two years and two centuries. As Malcolm and Malcolm point out it â€Å"adds to the sense of uncertainty and displacement† (1996: 85). The question of where the action takes place is more difficult to answer, but it will be discussed in the chapter â€Å"Dominica† of the seminar paper. Particular places of action re â€Å"Spanish Castle†, the â€Å"yellow- hot Market Street† (1 1), â€Å"the Imperial Road† (14fO and Cox's house. In the following chapters, those places will be analyzed in more detail. 2. Dominica Dominica plays an important role in the analysis of the setting, since it is probably the place where the entire plot takes place and it is furthermore the is land that accommodates the most important houses such as Ramage's Spanish Castle or Dr Cox's house. â€Å"Dominica is part of the Lesser Antilles in the Caribbean Sea, in a strip of islands known as the West Indies. It is situated between the French islands of Guadeloupe to he North and Martinique to the South. † (Kamyab 2009: 3). Other islands of the Lesser Antilles are Barbados and Trinidad (Brockhaus Weltatlas 1993: 255). arries Isla Harrington and at the end his wife is said to have stayed with relatives from Guadeloupe. As all these islands are directly mentioned, the reader can conclude that the location where the action takes place is a Caribbean island as well. Savory points out that Rosalie and Irene are â€Å"clearly in Roseau, Dominica in 1899† (2009:100). One fact which proves that Savory's statement is very likely to be true is the reoccurring motive of the â€Å"Imperial Road†. Along this new Imperial Road† (13) Ramage looks for a place to li ve. He finds his new home, a mansion called â€Å"Spanish Castle†.Another fact that may lead to the impression that Dominica is the place where the action takes place, is the â€Å"West Indian newspaper† (Rhys 1976: 12) which is mentioned in â€Å"Pioneers, Oh, Pioneers†: â€Å"the Dominica Herald and Leeward Islands Gazette†. First of all, the newspaper is named after Dominica, therefore the reader may conclude that this is the place where the story occurs. Furthermore, an article published in this fictional newspaper mentions the Imperial Road and defines ts idea as made â€Å"to attract young Englishmen with capital who would buy and develop properties in the interior† (19).The real Imperial Road had a similar concept. The administrator of Dominica at the period from 1899 to 1905, Henry Hesketh Bell, developed the idea of this concept. Bell was a fine publicist and attracted a number of new venturers on Crown Lands opened up by the Imperial Road , thirty on his own estimation, who together had invested about E40,OOO by the end of 1904 (Bell Papers (12. x. 1904)). In September 1900, while on leave in London, Bell wrote a long letter to The Times nder the title â€Å"Planting in Dominica,† extolling the virtues of the island and seeking young men.He received many replies, three planters returning with him to Dominica later that year (Hulme 2000:29). The house of the Cox family is described as one room which is: â€Å"C†¦ ] full of rockingchairs, a mahogany table, palm leafs fans, a tigerskin rug, family photographs, views of Bettws-y-Coed and a large picture of wounded soldiers in the snow, Napoleon's Retreat from MOSCOW' (Rhys 1979: 12). Furthermore, the fact that the two girls had to â€Å"go upstairs to bed† (13) leads to the conclusion that this house is not a simple one in the Caribbean.The financial situation of the Cox's is never directly mentioned in the text, but looking at the fact that they life in a house where, for example, timber-made chairs, belong to the furniture, the reader can come to the conclusion that the Cox's are a family that is well off. Here Rhys uses the setting to promote an â€Å"indirect characterization† (Toolan 1988: 104). To give some information about Dr Cox's character, a description of his desktop is used. â€Å"The Times weekly edition, the Cornhill Magazine, the Lancet and a West Indian newspaper, the Dominican Herald and Leeward Islands Gazette†(12), are to be found there.This indicates that Dr Cox is a man of vast reading and therefore well-educated. Objects like this â€Å"handled or props are two pictures. The first one is a picture of Bettws-y-Coed, this tells the reader that the Cox's have a British origin (http://www. betws-y-coed. com 2013). The second one with the historical Napoleonic background, implies that they are a family which is interested in culture and history. The house of the Cox family is situated near the ce ntre of the city, close to the Market Street, which is part of Irene's and Rosalie's way back home (11).The description of Dr Cox â€Å"sitting in an armchair with a hree- legged table by his side. On the table there were his pipe[†¦ ]. Also a Times weekly edition He was not to be spoken to (12) has also a biographical aspect. The place where Rhys's father used to sit is described in a similar way: a round table with a green- shaded reading lamp, the latest Times weekly edition a fortnight old, his pipe rack, and a large armchair where he sat reading and we weren't allowed to disturb him or speak to him (Rhys 1981: 68 f. ).Ramage's home is the place where the function of the setting, namely to underline the character traits, is most recognizable. Rhys focuses on â€Å"male exploitation of women, on women's resistance to and collusion with that exploitation, on marginalizes exiled fgures from the Third world, on class antagonisms and conflicts† (Malcolm ; Malcolm 1996: 1 1). But in â€Å"Pioneers, Oh, Pioneers† the protagonist is a â€Å"male outsider† (Malcolm ; Malcolm 1996: 82). The character Ramage is also not completely invented. The real Ramage was a well-known fgure in Dominica.His attempt to dig a hole to reach China stands in parody of Chamberlain and Bell's modernizing efforts: much digging to no ultimate purpose, and with moral laxity, madness, and death Hulme 2000: 10) The main intention of the fictional Ramage in â€Å"Pioneers, Oh, Pioneers† is to find peace: â€Å"Peace, that's what I am after. † (14) but the only way to find it is: † to pay for it' You'll be very much alone. † (14). But this does not bother Ramage much. He prefers being alone and is considered to be â€Å"very unsociable† (12). He does not follow any â€Å"invitations to dances, tennis parties and moonlight picnics† (1 2f).Furthermore he does not seem to be interested in religion very much, for he never goes to ch urch (13). The setting of the Imperial Road underlines this antisocial attitude. Only made â€Å"to attract young Englishmen† the Imperial Road is a very isolated place to live at. The closes neighbor of Ramage is the owner of Twickenham, Mr Eliot (14). In the beginning, Ramage appeals to the inhabitants of Dominica, whether they are black or white. He is â€Å"followed about by an admiring crowd of little Negro boys† (12) and regarded as â€Å"a gentleman† (12) by Miss Lambton. â€Å"Ramage appears made to fit in. † (Malcolm & Malcolm 1996: 88).It is conspicuous that Rhys uses the setting to create a link between the outward appearance of Ramage and the one of his house, the â€Å"Spanish Castle†. At the when it came to looks â€Å"(12), wearing his â€Å"tropical kit, white suit, red cummerbund, solar topee† (12). His eventual home, the Spanish Castle, is said to be â€Å"beautiful but not prosperous† (14). As the story continues, things change. Ramage is now â€Å"burnt a deep brown, his hair fell to his shoulders, his beard to his chest. † (16). With only â€Å"wearing sandals a leather belt, on one side which hung a cutlass, on the other a large pouch. (16), he attracts the attention of the other inhabitants of the island. 5 Spanish castle is now â€Å"unkempt deserted [†¦ ]. The grass on the lawn had grown very igh and the verandah hadn't been swept for days† (17). Rhys uses the setting to underline the change in Ramage's life. He doesn't care about how he looks like anymore and seems desperate, because he seems to be unable to catch up with the society on the island. He simply wants to find peace. He isolates himself from the island population and tries to go native, but fails. Ramage's public appearance as naked, with long hair and deep brown skin, seems to indicate a serious, if flawed, attempt to go native. Ramage looks – with the exception of the beard – Just like de pictions of the real natives of Dominica† (Hooper 2005: 125). Ramage may try to leave his British origin behind and to identify himself as a fixed part of the island. His whole struggle with trying to live a quiet life is reflected in the appearance of his estate. By marrying Isla, Ramage splits himself off from the English society and puts down roots on Dominica.But the people who live on the island do not except this marriage. He is said to be â€Å"lost to white society' (15) now. Resulting from his interracial marriage, the island population assume that he rejects the idea of the natural superiority of the British race (Wende 2012: 229). When Mrs Ramage disappears, rumors are spread that Ramage might have killed her. An article published in the Gazette calls him a â€Å"beastly murder[er]† (19). Toolan points out that there is a causal or analogical relation between the setting on the one hand and characters and events on the other hand (1988: 104).Dr Cox, who seem s to be the only friend of Ramage, is not able to see the danger signals. When he visits his friend, the last time before Ramage kills himself, he gives the impression to be okay. The â€Å"nasty beastly horrible Ramage. â€Å"(11) was now â€Å"wearing one of his linen suits, lean and pressed, and his hair and beard were trimmed. † (17). He says that he feels â€Å"splendid† (17) but his garden tells the truth. Here the setting, in this case the garden, gives some information about the feelings of the male main protagonist.As already mentioned above, the garden is â€Å"unkempt and deserted The grass on the lawn had grown very high and the verandah hadn't been swept for days. † (17). Deep inside he also feels â€Å"unkempt and deserted† (17) because the people throw stones at his house (18), think that he killed his wife (19) and do not accept his new way of dressing 16) and his marriage with Isla (15). That fthe servants have all walked out† (17 ) shows that everyone left Ramage and he is all alone now, as Dr Cox predicted it before. Although he said at the beginning that he had no problem with being alone (14), he is hapless now.Nobody seems to understand him and therefore he is not able to find the only thing he was after: â€Å"Peace† (14). Neither fitting into black nor white society, Ramage decides to commit suicide. On the day of his funeral â€Å"it was dream. 6 The comparison of the Imperial Road and the Market Street is used to clarify the function of the etting to underline the racial segregation. â€Å"In Pioneers, Oh, Pioneers,†the two contrasting worlds meet physically on Market Street in the form of black and white women there† (Malcolm ; Malcolm 1996: 85). Rhys illustrates the complex hierarchy of race and class (Davis 2005) in her short story.She does this by using the setting. Like Davis points out, on Market Street â€Å"the black women were barefooted, wore gaily striped turbans and highwaisted dresses† (Rhys 1976: 1 1), while Afro-French Madame Menzies maintained the dignity of her old-fashioned riding habit, and British Mr. Ramage lived out an imperialist antasy in his â€Å"tropical kit, white suit, red cummerbund, solar topee† (12). These different perspectives are linked through the consciousness of the young protagonist who rejects the reductive, hegemonic vision of colonial society (Davis 2005).When Ramage arrives on the island, he is looking for an estate and he tells Dr Cox that he â€Å"was told that there were several places going along this new Imperial Road [†¦ ]† (13). But Dr Cox is not convinced of this plan. ANon't last. † (13), he says. But Ramage still decides to buy an estate located at this lonely road. He acquires Spanish Castle, â€Å"one of the older properties† (14). The nearest estate is the one of Mr Eliot. It was used to be called â€Å"Malgr © Tout†, but now it is called â€Å"Twickenha m† (14) after a London suburb.Wende points out that this renaming represents the British sovereignty (2012: 227). In contrast to the young men, like â€Å"young Errington, young Kellaway, who had bought estates along the Imperial Road and worked hard C†¦ ]† (14) but failed and had to sell their land after only a few time had passed, Ramage does not want to gain prosperity. The only thing he wants to find is peace (14). In one way this can be interpreted as a â€Å"desired escape from Britain† (Hooper 2005: 124). The loud and crowded Market Street corresponds to the lonely Imperial Road.The Imperial Road seems to be a place built up by white people to separate themselves from the black society. It is a place where people who want to live in another country, but do not want to integrate, live. They rather want to stay among people of the same origin. This is to be noticed by the fact that there are no black people to be found on the Imperial Road. Only rich wh ite people live there. The Market Street is the place where black as well as white people meet. As already mentioned above: â€Å"[T]he few white women carried parasols. The black women were barefoot, wore gaily striped turbans and highwaisted dresses. (11) Though they are humans, all people Rosalie watches on Market Street are only part of the setting (as well as 7 the admiring negro crowd). They are used to underline the contrast between black and white on the island, and are not important as characters for the story. They are only props, as Chatman defines (1993: 63). The white women do not seem to enjoy the weather. Their appearance is much darker than the one of the black women, who barefooted, but Mrs Menzies also passes by riding, she is therefore in the physical osition to look down on other people.This underlines the clear distance between the colonialists and the people who are colonialized. Even though slavery was abolished by this time, hierarchical structures were stil l present (Wende 2012: 237). In â€Å"Pioneers, Oh, Pioneers† the white society seems to be very hostile to acts or people who do not fit in their idea of life. Not only the Market Street shows the racial segregation, but also the description of the two cemeteries. On the one hand, there is â€Å"the Catholic cemetery, where all day the candles burnt almost invisible in the unlight.When night came they twinkled like fireflies. The graves were covered with flowers- some real, some red of yellow paper or little gold cut-outs. † (21). This cemetery is the one where mostly black people were buried. Just like their clothes, their cemetery is described in a more colorful way. Whereas â€Å"the Anglican cemetery, which was not very far away, down the hill, was deserted and silent. † (21). Just like the people, the cemeteries have some point where they seem to be alike, for they both are places where people are buried, but they have significant differences.When eferring to the people this could be interpreted as the black people are more kindly (bright) and the white are more dismissive (grey). 3. Conclusion An impoverished stranger comes from off the island, usually from ‘home', and courts and marries a local woman who, in narrative terms, is seen to represent the island itself. That story is fundamental to Rhys's work. [†¦ ] It is the story of Mr. Ramage, in â€Å"Pioneers, Oh Pioneers. † And, crucially, it is the story of Rhys herself (Hulme 2000: 20). In Jean Rhys's short story the setting fulfills several functions.One the one hand, it is sed to determine the place where the action takes place. As already mentioned above, the place where the entire plot takes place and where the houses which are directly mentioned, such as Ramage's Spanish Castle or Dr Cox's house, are to be found, is Dominica. On the other hand, the setting is used to characterize acting people in an indirec way. For example, it is never said that the Cox f amily is a rich one. Only the description of one room in their house with chairs made of timber and a tigerskin rug leads the reader to the conclusion that they are a family which is well off.Furthermore, the setting tells the reader important character traits of 8 Dr Cox. The description of his desktop with a lot of newspapers implies that he is intelligent and a prestigious man. Another function of the setting in â€Å"Pioneers, Oh, Pioneers† is to point out the racial segregation, that was still present on Dominica in 1899. Although the differences were never mentioned explicitly, it becomes clear that there is a huge discrepancy between black and white people, as the setting implies. For example, when Ramage marries Isla, he is â€Å"lost to white society' (15).In their eyes, the way Ramage lives does ot match their ideas of a hierarchy everyone has to stick to. They want a clear separation of colonialists and the people who are colonialized. As Malcolm and Malcolm point out â€Å"Pioneers, Oh, Pioneer†, deals with the cultural clash between those two groups (1996: 83). The white people outrank the black islanders. This is already got to know. The white women Mrs Menzies does not only feel like she is in a higher position, she actually really is, because she rides past the black people on her horse. Furthermore Malcolm and Malcolm emphasize that even the date emphasizes the clash.Peter Hulme sees in Jean Rhys's short story ‘Pioneers, Oh Pioneers' not only a foreshadowing of the life of the inexperienced settler, but a critique of precisely the sorts of imperial road-building ambitions that were promoted by politicians and administrators such as Chamberlain and Hesketh Bell.

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